Google has appealed against a record €2.4bn antitrust fine imposed by the European Commission in June.
The EU fined the internet giant for abusing its dominance in Europe to position its own shopping comparison service at the top of Google search results.
Margrethe Vestager, the EU’s competition commissioner had said that Google's activity was "illegal under EU antitrust rules".
The Commission ordered Google to stop its practice of promoting its own shopping comparison service above rivals by 28 September.
The company was expected to appeal the fine, which was the largest ever penalty issued by the regulator, after saying it had "respectfully disagreed" with the ruling.
It is anticipated to take years before the Luxembourg-based General Court rules on the case.
Google has not asked for an interim order to suspend the EU decision, a court spokesperson told Reuters.
Another spokesperson said the EU competition enforcer will defend its decision in court.
Google said declined to comment further.
Shares in Alphabet rose 1.07% to US$951.46 each in early US trading.